![]() Neuroscience 280:142–155Ĭeruti S, Villa G, Genovese T, Mazzon E, Longhi R, Rosa P, Bramanti P, Cuzzocrea S, Abbracchio MP (2009) The P2Y-like receptor GPR17 as a sensor of damage and a new potential target in spinal cord injury. Neurosurgery 67:1733–1744īray AF, Cevallos RR, Gazarian K, Lamas M (2014) Human dental pulp stem cells respond to cues from the rat retina and differentiate to express the retinal neuronal marker rhodopsin. J Neurotrauma 12:1–21īozkurt G, Mothe AJ, Zahir T, Kim H, Shoichet MS, Tator CH (2010) Chitosan channels containing spinal cord-derived stem/progenitor cells for repair of subacute spinal cord injury in the rat. Stem Cells 26:1787–1795īasso DM, Beattie MS, Bresnahan JC (1995) A sensitive and reliable locomotor rating scale for open field testing in rats. Transplantation of DPSCs might therefore be a suitable candidate for treating SCI and other neuronal degenerative diseases.Īrthur A, Rychkov G, Shi S, Koblar SA, Gronthos S (2008) Adult human dental pulp stem cells differentiate toward functionally active neurons under appropriate environmental cues. ![]() Thus, chitosan scaffolds were non-cytotoxic and provided a conducive and favorable microenvironment for the survival and neural differentiation of DPSCs. Transplantation of DPSCs together with chitosan scaffolds into an SCI rat model resulted in the marked recovery of hind limb locomotor functions. The Wnt/β-catenin signaling pathway played a key role in the neural differentiation of DPSCs combined with chitosan scaffolds. Compared with the control group, the levels of BDNF, GDNF, b-NGF, and NT-3 were significantly increased in the DPSC/chitosan-scaffold group. Our study revealed a higher cell viability and neural differentiation in the DPSC/chitosan-scaffold group. DPSCs incubated with chitosan scaffolds were treated with neural differentiation medium for 14 days and then neural genes and protein markers were analyzed by Western blot and reverse transcription plus the polymerase chain reaction. Cell viability and the secretion of neurotrophic factors were analyzed. DPSCs were incubated with chitosan scaffolds. The purpose of this study is to investigate the role of chitosan scaffolds in the neural differentiation of DPSCs in vitro and to assess the supportive effects of chitosan scaffolds in an animal model of SCI. ![]() Human dental pulp stem cells (DPSCs) are abundant available stem cells with low immunological incompatibility and can be considered for cell replacement therapy. Chitosan scaffolds have therapeutic benefits for spinal cord regeneration. Cell-based transplantation strategies hold great potential for spinal cord injury (SCI) repair. ![]()
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